Isolation of alkylated rat hemoglobin adducts using metal-affinity chromatography |
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| Authors: | Ya A Dubrovskii V D Gladilovich I A Krasnov E P Podolskaya E A Murashko V N Babakov |
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| Institution: | 1.Research Institute of Human Hygiene,Occupational Pathology and Ecology,Leningrad oblast,Russia;2.Institute for Analytical Instrumentation,Russian Academy of Sciences,St. Petersburg,Russia |
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| Abstract: | Metal-affinity chromatography with Cu2+ containing sorbent was used for separation of globin peptides alkylated by sulfur mustard. It was shown that matrix-assisted
laser desorption/ionization time-of-flight mass spectrometry allowed isolating peptides alkylated by sulfur mustard (HD) at
cysteine-126, -94 and glutamic acid-27 with MH+ of 1444.62, 1561.66, 1676.78 Da, respectively, from rat globin tryptic digest incubated with 60 μM of HD. An alkylated peptide
with MH+ of 1444.63 Da was isolated from globin hydrolyzate incubated with 3 μM of HD. |
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