NCI-H716 cells as a model for endocrine differentiation in colorectal cancer |
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Authors: | Adriaan P de Bruïne Winand N M Dinjens Margriet M J Pijls Edith P M v d Linden Mat J M Rousch Peter T Moerkerk Antony F P M de Goeij Fred T Bosnian |
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Institution: | 1. Department of Pathology, University Hospital Maastricht, Rotterdam, The Netherlands 2. Department of Pathology, Erasmus University, Rotterdam, The Netherlands
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Abstract: | In colonic neoplasms, endocrine differentiation is encountered not only in carcinoid tumors but also in adenocarcinomas, where
endocrine cells may represent a distinct line of differentiation in the tumor. The significance of endocrine differentiation
in colorectal cancer is not well established, partly because of the paucity of tumor cell lines which can serve as a model
for studying endocrine differentiation. In this report we describe the properties of NCI-H716 cells, a cell line derived from
a poorly differentiated adenocarcinoma of the caecum, under various in vitro conditions and as xenografts in athymic mice.
Phenotypical properties were immunohistochemically assessed using a panel of differentiation related antibodies, and also
by Northern blot analysis and by electron microscopy. Receptors for biogenic amines and peptide hormones were analyzed by
ligand binding assay. These studies show that:
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NCI-H716 cells can be undifferentiated, or show endocrine, mucin-producing or “amphicrine” properties.
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Endocrine differentiation of NCI-H716 cells preferentially occurs in xenografts in athymic mice, which suggests that mesenchymal
elements induce endocrine differentiation.
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NCI-H716 cells express large amounts of high affinity receptors for gastrin, serotonin and somatostatin and these substances
can regulate growth. Thus, NCI-H716 cells form a suitable model for the study of endocrine differentiation in intestinal epithelium
and of auto- or paracrine growth regulation in intestinal neoplasia.
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Keywords: | Colorectal carcinoma Cell line Endocrine Differentiation Induction Receptor |
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