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Polyamines in the photosynthetic apparatus
Authors:Kiriakos Kotzabasis  Constantina Fotinou  Kalliopi A Roubelakis-Angelakis  Demetrios Ghanotakis
Institution:(1) Department of Biology, University of Crete, Heraklion, Crete, Greece;(2) Department of Chemistry, University of Crete, Heraklion, Crete, Greece;(3) Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion, Crete, Greece
Abstract:The three main polyamines putrescine (Put), spermidine (Spd) and spermine (Spm) were characterized by HPLC in intact spinach leaf cells, intact chloroplasts, thylakoid membranes, Photosystem II membranes, the light-harvesting complex and the PS II complex. All contain the three polyamines in various ratios; the HPLC polyamine profiles of highly resolved PS II species (a Photosystem II core and the rection center) suggest an enrichment in the polyamine Spm.Abbreviations Chl chlorophyll - HPLC high performance liquid chromatography - LHC light-harvesting complex - PS II Photosystem II - PS II-RC Photosystem II reaction center - Put putrescine - Spd spermidine - Spm spermine - 10%S-core D1-D2-Cyt b559-47 kD-43 kD complex
Keywords:polyamines  thylakoids  Photosystem II
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