响应曲面法优化灵芝廉价型深层发酵培养基的研究

为了获得生产用廉价型灵芝发酵培养基,采用中心组合旋转设计法和响应曲面法对低成本培养基组分进行了优化。优化的四个组分为玉米粉(x1)、麸皮粉(x2)、豆饼粉(x3)和蔗糖(x4)。结果表明,灵芝菌体发酵和多糖发酵的培养基预测模型分别为:Y1=15.1–0.31x1–0.34x2+0.36x3–0.44x4–1.26x12–1.98x22–0.85x32–1.15x42–0.59x2x3和Y2=2.0–0.08x1–0.08x2+0.04x3–0.09x4–1.13x12–0.33x22–0.08x32–0.16x42–0.16x2x3–0.10x1x4。从中获得菌体发酵的最优配方为:玉米粉19.7g/L,麸皮粉11.3g/L,豆饼粉6.3g/L,蔗糖19.5g/L;多糖发酵的最优配方为:玉米粉19.6g/L,麸皮粉11.0g/L,豆饼粉6.7g/L,蔗糖19.1g/L。150L发酵罐中试放大结果表明,灵芝菌体的产量为16.92g/L,多糖产量为1.86g/L。所得培养基为灵芝产品的高效低成本生产提供了基础。

Optimization of low-cost medium constituents for submerged fermentation of Ganoderma lucidum by using response surface methodology

To obtain a low-cost medium for submerged fermentation of Ganoderma lucidum in a large scale, four medium constituents were optimized by using central composite rotatable design (CCRD) and response surface methodology (RSM). The four factors were corn flour (x_1), wheat bran flour (x_2), soybean flour (x3) and sucrose (x_4). The results show that the mathematical model for biomass production and polysaccharide production are Y_1 = 15.1 - 0.31x_1 - 0.34x_2 + 0.36x_3 - 0.44x_4 - 1.26x_1~2 - 1.98x_2_2 - 0.85x_3~2 - 1.15x_4~2 - 0.59x_2x_3, and Y_2 = 2,0 - 0.08x_1 - 0.08x_2 + 0.04x_3 - 0.09x_4 - 1.13x_1~2 - 0.33x_2~2 - 0.08x_3~2 - 0.16x_4~2 - 0.16x_2x_3 - 0.10x_1x_4, respectively. Based on the models, the optimum medium for biomass production is corn flour 19.7g/L, wheat bran flour 11.3g/L, soybean flour 6.3g/L, and sucrose 19.5g/L; while the optimum medium for polysaccharide production is corn flour 19.6g/L, wheat bran flour 11.0g/L, soybean flour 6.7g/L, and sucrose 19.1g/L. The maximum biomass and polysaccharide in a 150-L stirred-tank bioreactor reach 16.92g/L and 1.86g/L under the optimized medium, respectively. The use of the medium can reduce the costs for both biomass and polysaccharide production by G. lucidum at large scale.