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Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
Authors:Boris Shklyar  Jeny Shklover  Estee Kurant
Affiliation:1.Department of Anatomy and Cell Biology and the Rappaport Institute for Research in the Medical Sciences, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology
Abstract:The proper elimination of unwanted or aberrant cells through apoptosis and subsequent phagocytosis (apoptotic cell clearance) is crucial for normal development in all metazoan organisms. Apoptotic cell clearance is a highly dynamic process intimately associated with cell death; unengulfed apoptotic cells are barely seen in vivo under normal conditions. In order to understand the different steps of apoptotic cell clearance and to compare ''professional'' phagocytes - macrophages and dendritic cells to ''non-professional'' - tissue-resident neighboring cells, in vivo live imaging of the process is extremely valuable. Here we describe a protocol for studying apoptotic cell clearance in live Drosophila embryos. To follow the dynamics of different steps in phagocytosis we use specific markers for apoptotic cells and phagocytes. In addition, we can monitor two phagocyte systems in parallel: ''professional'' macrophages and ''semi-professional'' glia in the developing central nervous system (CNS). The method described here employs the Drosophila embryo as an excellent model for real time studies of apoptotic cell clearance.
Keywords:Developmental Biology   Issue 78   Cellular Biology   Molecular Biology   Genetics   Bioengineering   Drosophila   Immunity   Innate   Phagocytosis   Apoptosis   Genes   Developmental   Cell Biology   biology (general)   genetics (animal and plant)   life sciences   embryo   glia   fruit fly   animal model
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