Transmission of organelle genomes in citrus somatic hybrids |
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Authors: | Moreira Cristina D Chase Christine D Gmitter Fred G Grosser Jude W |
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Institution: | (1) Institute of Food and Agricultural Sciences, Horticultural Sciences Dept., University of Florida, P.O. Box 110690, USA, Gainesville, FL 32611-0690, USA;(2) Institute of Food and Agricultural Sciences, CREC, University of Florida, P.O. Box 1088, Lake Alfred, FL 33850, USA |
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Abstract: | Restriction fragment length polymorphisms (RFLPs), were used to analyze the organelle composition of two-year-old trees, recovered
from two different experiments: protoplasts from embryogenic cell suspensions of `Succari' sweet orange (C. sinensis L. Osbeck) were fused with leaf protoplasts of Citropsis gilletiana Swingle & M. Kell or to leaf protoplasts of Atalantia ceylanica(Arn.) Oliv. The somatic hybrids of both fusion combinations had the mitochondrial genome from the embryogenic partner. In
some somatic hybrids, non-parental fragments were observed among the mitochondrial patterns. Somatic hybrids between `Succari'
+ Atalantia had plastid DNA from the embryogenic parent, while the somatic hybrids of `Succari' + Citropsis all had both parental chloroplast genomes. The relative abundance of organelle DNAs in the donor embryogenic and leaf cells
may explain the consistent transmission of the embryogenic parent mitochondrial DNA and the inheritance of the chloroplast
genome from either parent.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | chloroplast DNA citrus mitochondrial DNA RFLPs somatic hybrids |
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