Synergistic interaction between coumarin 1,2-benzopyrone and indole-3-butyric acid in stimulating adventitious root formation in Vigna radiata (L.) Wilczek cuttings: I. Endogenous free and conjugated IAA and basic isoperoxidases |
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Authors: | Kamel Tartoura Andrea da Rocha Sahar Youssef |
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Institution: | (1) Department of Agricultural Botany, Suez-Canal University, Ismailia, Egypt;(2) Department of Plant Pathology, Michigan State University, 62 Plant Biology Building, East Lansing, MI 48824, USA |
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Abstract: | Cuttings from 7-day-old Vigna radiata seedlings were treated for 24 h with various concentrations of coumarin and/or indole-3-butyric acid (IBA), applied either
alone or in combination, in order to stimulate adventitious root formation (ARF). The effects of treatment on endogenous free
and conjugated indole-3-acetic acid (IAA), basic peroxidase (basic PER) activity and its isoperoxidases analysis and their
relation to ARF were then investigated at the potential rooting sites during the first 96 h after application. Simultaneously,
combined treatments acted synergistically in inducing more adventitious roots in treated cuttings than in those treated with
coumarin or IBA individually, as compared with the control. Endogenous free IAA increased transiently in treated cuttings
as compared with the control and the maximum increase occurred with the combined treatment. This suggests that coumarin and
IBA may act synergistically in increasing the endogenous free IAA level during the induction phase of rooting to initiate
more roots. Likewise, higher level of conjugated IAA was also found in treated cuttings than in untreated ones, during the
primary events of ARF, with the maximum level occurring in the combined treatment. Comparison of the dynamics of conjugated
IAA and activity of basic PERs led to conclusion that the former but not the latter is responsible for downregulation of endogenous
IAA levels significantly during the primary events of ARF. A sharp increases in basic PERs occurred during the secondary events
of ARF, suggesting their role in root initiation and development rather than root induction. |
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Keywords: | Adventitious root formation Basic isoperoxidases Coumarin Endogenous free and conjugated IAA Indole-3-butyric acid Mung bean cuttings |
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