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Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP |
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| Authors: | Shima Kensuke Kawamura Naohisa Hinenoya Atsushi Sugimoto Norihiko Wu Yuluo Asakura Masahiro Nishimura Kazuhiko Nair Gopinath B Yamasaki Shinji |
| Affiliation: | Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka, Japan. |
| Abstract: | Using culture-independent technology, PCR-RFLP were used to identify and type STEC in the stool of a patient with HUS. Fecal PCR-RFLP patterns were identical to those of the STEC O157:H7 isolated from the patient. |
| Keywords: | Hemolytic uremic syndrome restriction fragment length polymorphisms Shiga toxin-producing Escherichia coli |
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