| 固氮酶铁钼辅基在分离纯化中结构变化的新证据 |
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| 引用本文： | 张凤章,黄河清,龙敏南,邱雪慧,许良树.固氮酶铁钼辅基在分离纯化中结构变化的新证据[J].中国生物化学与分子生物学报,1999,15(2):328-331. |
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| 作者姓名： | 张凤章 黄河清 龙敏南 邱雪慧 许良树 |
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| 作者单位： | 厦门大学生物学系 |
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| 摘 要： | 根据Ｋｉｍ－Ｒｅｅｓ模型［１］，固氮酶铁钼辅基（即ＦｅＭｏｃｏ或Ｍ簇），是由一个ＭｏＦｅ３Ｓ３簇和一个Ｆｅ４Ｓ３簇通过三个Ｓ－桥联接而成．然而，自Ｓｈａｈ等（１９７７）首次从结晶的钼铁蛋白中分离出具有生物重组活性的ＦｅＭｏｃｏ以来，固氮研究者们一直致...
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| 收稿时间： | 1999-04-20 |
New Evidence for Structure Change of Iron molybdenum Cofactor from Nitrogenase MoFe Protein in Separation |
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| ZHANG Fengzhang,HUAN Heqing,LONG Minnan,QIU Xuehui,XU Liangshu.New Evidence for Structure Change of Iron molybdenum Cofactor from Nitrogenase MoFe Protein in Separation[J].Chinese Journal of Biochemistry and Molecular Biology,1999,15(2):328-331. |
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| Authors: | ZHANG Fengzhang HUAN Heqing LONG Minnan QIU Xuehui XU Liangshu |
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| Institution: | (Department of Biology,Xiamen University,Xiamen 361005 |
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| Abstract: | The iron molybdenum cofactor (FeMoco) of nitrogenase MoFe protein from Azotobacter Vinelandii OP was extracted by N methylformamide (NMF).Effects of FeMoco(in NMF) on electronic spectrum and fluorescence intensity (in 1 mol/L NaOH) were investigated by fluorophotometric titrations and compared with results of effects of (NH 4) 2MoS 4 and its complexes with Na 2S,Na 2S 2 and (NH 4) 2S X on relative properties of FDMA.It was found that titration curve for quenching of FDMA with FeMoco was very similar to that for quenching of FDMA with complexes of (NH 4) 2MoS 4 and Na 2S 2(1∶3,mol/mol).The results showed that FeMoco(N) probably contained S S bonds and Its structure was found to be a changing one. |
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| Keywords: | Nitrogenase Iron molybdenum cofactor Fluorophotometric titration Structural change |
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