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| RP-HPLC法同时测定十种五加属植物叶中三萜Chiisanoside和Chiisaogenin | |
| 引用本文： | 刘向前,戴玲,戴秀珍.RP-HPLC法同时测定十种五加属植物叶中三萜Chiisanoside和Chiisaogenin[J].天然产物研究与开发,2013,25(1):71-75. |
| 作者姓名： | 刘向前 戴玲 戴秀珍 |
| 作者单位： | 1. 湖南省中药现代化研究重点实验室湖南中医药大学药学院,长沙410208;中南大学制药工程系,长沙410083 2. 湖南省中药现代化研究重点实验室湖南中医药大学药学院,长沙,410208 3. 中南大学制药工程系,长沙,410083 |
| 基金项目： | Hunan Provincial Natural Science Foundation(11JJ2042);Science of Pharmaceutical Analysis of Twelfth Five-Year Key Discipline Projects of Hunan University of Chinese Medicine;Innovation Platform and Open Foundation Project of Higher Colleges of Hunan Province(11K048);Changsha City Science and Technology Bureau Key Projects(K1104104-21) |
| 摘 要： | 建立反相高效液相色谱法(RP-HPLC)测定chiisanoside和chiisanogenin的定量分析方法,利用最优色谱条件对十种中韩五加属植物叶中的三萜chiisanoside和chiisanogenin进行定量分析.研究结果表明,最佳色谱条件为:ODS-C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水(55:45);检测波长:205 nm;柱温:40℃;流速:1.0 mL/min.Chiisanoside和chiisanogenin线性范围和回归方程分别为16.5～ 181.5 μg/mL,Y=5498356X-9738(r=0.9995)和4.88～78.0 μg/mL,Y=5753131X-8289 (r=0.9979),加样回收率分别为98.87％(标准偏差为1.14％)和98.83％(标准偏差为0.72％). |
| 关 键 词： | 五加属 chiisanoside chiisanogenin 反相高效液相色谱法 |
Simultaneous Determination of Chiisanoside and Chiisanogenin from Leaves of Ten Kinds of Acanthopanax Miq.Plants by RP-HPLC |
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| LIU Xiang-qian , DAI Ling , DAI Xiu-zhen.Simultaneous Determination of Chiisanoside and Chiisanogenin from Leaves of Ten Kinds of Acanthopanax Miq.Plants by RP-HPLC[J].Natural Product Research and Development,2013,25(1):71-75. | |
| Authors: | LIU Xiang-qian DAI Ling DAI Xiu-zhen |
| Institution: | 1Hunan Key Laboratory of Traditional Chinese Medicine modernization,School of Pharmacy,Hunan University of Chinese Medicine, Changsha 410208,China;2School of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China |
| Abstract: | |
| Keywords: | Acanthopanax Miq chiisanoside chiisanogenin RP-HPLC |
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