| 扬子鳄红细胞超氧物歧化酶的纯化及其性质研究 |
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| 引用本文： | 周衍茂,尹路明. 扬子鳄红细胞超氧物歧化酶的纯化及其性质研究[J]. Acta biochimica et biophysica Sinica, 1994, 0(6) |
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| 作者姓名： | 周衍茂 尹路明 |
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| 作者单位： | 安徽教育学院生物系，中国科学技术大学生物系 |
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| 摘 要： | 扬子鳄红细胞超氧物歧化酶的纯化及其性质研究周衍茂（安徽教育学院生物系，合肥２３００６１）尹路明（中国科学技术大学生物系，合肥２３００２６）关键词超氧物歧化酶；纯化；扬子鳄；红细胞超氧物歧化酶（的田广泛存在于各类生物组织中，是生物体内超氧自由基有效的清...
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| 关 键 词： | 超氧物歧化酶；纯化；扬子鳄；红细胞 |
Purification and Characterization of Superoxide Dismutase from the Erythrocytes of Chinese Alligator |
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| ZHOU Yan-Mao. Purification and Characterization of Superoxide Dismutase from the Erythrocytes of Chinese Alligator[J]. Acta biochimica et biophysica Sinica, 1994, 0(6) |
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| Authors: | ZHOU Yan-Mao |
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| Abstract: | Copper-zine superoxide dismutase(SOD)from the erythrocytes of Chinese alligator has been purified by treatment with ethanol-chloroform mixture,K2HPO4 fractional salting-out, actone precipitation and DEAE-Sephadex A-50 column chromatography.Staining for SOD activity and by coomassie brilliant blue of the PAGE pattern of Purified SOD exhibits a single band.The molecular weight of purified SOD is about 32.8 kd as determined by gel filtration on Sephadex G-75 and its subunit molecular weight is about 16.2 kd as assayed with SDS-PAGE.It may be inferred that the enzyme is composed of two subunits of identical size.The enzyme subunit consists of about 153 amino acid residues,from which trypophan is absent. |
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| Keywords: | Superoxide dismutase Purification Chinese alligator Erythrocyte |
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