水稻草矮病毒在水稻原生质体中的表达

通过建立水稻原生质体培养体系，经多聚鸟氨酸（PLO）介导将提纯的水稻草矮病毒（Rice grassy stunt virus，RGSV）接种到水稻原生质体内，利用酶联免疫吸附法（ELISA）及蛋白免疫印迹法（Western blot），研究RGSV在水稻原生质体内的生长周期及其编码蛋白的表达情况。结果表明： RGSV在接种后24h左右开始在原生质体内复制，36h左右达到最大值。NS6在15h左右开始表达，在30h左右达到最大值。

Expression of Rice Grassy Stunt Virus in Rice Protoplasts

With the rice protoplast infection system, the purified virus particles of Rice grassy stunt virus were delivered into rice protoplasts derived from suspension cells of rice cultivars Tainong 67 via PLO-mediated method. Using antisera prepared against the purified virus particles of RGSV, NS3 fusion protein and purified NS6 protein, expressison profiles of RGSV and its encoded proteins in rice protoplasts were investigated by indirect ELISA and Western blot. The results showed as follows: RGSV was detectable in rice protoplasts 24 h after inoculation, and reached its highest level 36 h after inoculation. NS6 was detected 15 h after inoculation, and reached its highest level 30 h after inoculation.