Plant Regeneration from Immature Zygotic Embryo-Derived Embryogenic Calluses and Cell Suspension Cultures of Catharanthus roseus |
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Authors: | Kim Suk Weon In Dong Su Choi Pil Son Liu Jang R |
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Institution: | (1) Laboratory of Plant Genomics Services, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yuseong, Daejeon, 305-600, South Korea;(2) Laboratory of Functional Genomics for Plant Secondary Metabolism (National Research Laboratory), Eugentech Inc., P.O. Box 115, Yuseong, Daejeon, 305-333, South Korea |
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Abstract: | Culture conditions for plant regeneration in immature zygotic embryo-derived embryogenic cell suspension cultures of Catharanthus roseus (Madagascar periwinkle) Little Bright Eye are described. Immature zygotic embryos formed off-white, friable calluses at a frequency of 20% on Murashige and Skoog (MS) medium supplemented with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D) after 8 weeks of culture. After a second subculture using MS basal medium at 4-week intervals, off-white friable calluses formed a small quantity of yellowish, compact embryogenic calluses. Upon transfer to MS basal medium, embryogenic calluses gave rise to numerous somatic embryos. Cell suspension cultures were established with embryogenic calluses using liquid MS medium supplemented with 4.52 µM 2,4-D. Embryogenic cell clumps from cell suspension cultures developed into plantlets at a frequency of 56.7% when plated onto MS basal medium. Plantlets were transplanted to potting soil and grown to maturity in a growth chamber. |
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Keywords: | Apocynaceae immature zygotic embryo Madagascar periwinkle somatic embryo somatic embryogenesis |
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