DNA topoisomerase activities in concanavalin A-stimulated lymphocytes |
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| Authors: | G Taudou G Mirambeau C Lavenot A der Garabedian J Vermeersch M Duguet |
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| Institution: | 1. Service de Physiopathologie de l''Immunité, Institut Pasteur, 25, rue du Dr. Roux. 75 724 Paris Cedex 15, France;2. Laboratoired''Enzymologie des Acides Nucleiques, Université Pierre et Marie Curie, 96, Bd. Raspail, 75 006 Paris, France |
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| Abstract: | Topoisomerase activities have been measured in nuclear extracts of concanavalin A-stimulated lymphocytes. In parallel with the wave of DNA synthesis, type II topoisomerase activity was considerably increased. After 72 h treatment, this activity was stimulated approx. 20-fold over the activity in untreated cells. In contrast, type I topoisomerase was poorly stimulated after 24 h treatment, and 4-5-fold after 72 h. These findings, together with our previous results on regenerating rat liver, suggest a major role of topoisomerase II in DNA replication. |
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| Keywords: | Mitogenic agent DNA replication Lymphocyte Topoisomerase Con A concanavalin A EGF epidermal growth factor |
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