Bifidobacterium bifidum monoassociation of gnotobiotic mice: Effect on enterocyte brush-border enzymes |
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Authors: | H Kozáková Z ?eháková J Kolínská |
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Institution: | (1) Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 549 22 Novy Hrádek, Czechia;(2) Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia |
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Abstract: | The effect of intestinal colonization withBifidobacterium bifidum (Gram-positive anaerobic bacterium colonizing the intestine of healthy new-born mammals, exhibiting a probiotic effect, protecting
the intestinal mucosa against colonization by pathogenic microflora) on enterocyte brush-border enzymes was examined in weaned
23-d- and in 2-month-old gnotobiotic inbred mice and compared with that in corresponding germ-free (GF) and conventional (CV)
controls. The two groups of GF mice were associated with humanB. bifidum 11 d before the end of the experiment. Specific activity of enterocyte brush-border enzymes—lactase, alkaline phosphatase
and γ-glutamyltranspeptidase was significantly higher in both age groups of GF mice in comparison with CV ones; on the other
hand, sucrase and glucoamylase activities were higher in CV mice. Monoassociation withB. bifidum accelerates biochemical maturation of enterocytes resulting in a shift of specific activities of brush-border enzymes between
the values found for GF and CV mice. This effect ofB. bifidum supplementation was less pronounced for alkaline phosphatase, sucrase, glucoamylase and dipeptidyl peptidase IV in immature
gut of weaned mice than of 2-month-old ones. |
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