维甲酸与星状孢子素联合分化诱导HL-60细胞过程中酪氨酸蛋白质磷酸化系统的变化

以人早幼粒白血病细胞株（HL-60）为材料，对维甲酸与星状孢子素联合诱导HL-60细胞过程中胞浆和膜部分的蛋白质酪氨酸磷酸化水平变化进行了研究．结果表明，诱导后2～24h范围内，TPK活力出现波动，随时间延长（24～27h），胞浆部分TPK活力下降，膜溶脱部分TPK活力上升；PTPP活力明显升高且上升幅度比TPK大得多．利用抗P-tyr-BSA抗体分析底物含量变化的结果与相应时相的TPK和PTPP活力变化趋势一致．

Alternations of p-tyr-protein System during the Differentiation of Human Promyelotic Leukemia Cells (HL-60) Induced by RA and Staurosporine

Alternations of TPK、PTPP activities in cytosol and membranous fractions and P-tyrprotein content of cytosol fraction were studied during the differentiation of HL-60 cells induced by RA and staurosporine. It was found that the TPK activity showed a fluctuation in the early stage of differentiation (2～24h). In a later time,the TPK activity declined in cytosol fraction and increased in membranous fraction. The PTPP activity in both fraction increased singificantly.The antui-phosphotyrosine-protein antibody was used to study the p-tyr-protein content of soluble fraction of HL-60 cells treated with RA and staurosporine. The result showed that the change of P-tyr-protein content was the same with the alternations of TPK and PTPP activities.