Compartment analysis of plant cells by means of turgor pressure relaxation: II. Experimental results onChara corallina |
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Authors: | Stephan Wendler Ulrich Zimmermann |
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Institution: | (1) Arbeitsgruppe Membranforschung am Institut für Medizin der Kernforschungsanlage Jülich, 5170 Jülich, Federal Republic of Germany;(2) Lehrstuhl für Biotechnologie der Universität Würzburg, 8700 Würzburg, Federal Republic of Germany |
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Abstract: | Summary The three-compartment model (paper I) described turgor pressure relaxations as a sum of two exponential functions. The predicted shape of the curves could be confirmed inChara corallina by improving the recording and processing of data. An evaluation on the basis of the three-compartment model provided values for the hydraulic conductivity of the plasmalemma ofLp
p=2×10–5 to 4×10–5 cm sec–1 bar–1 and ofLp
i=3×10–5 to 1×10–4 cm sec–1 bar–1 for the tonoplast (assuming the area to be 90% of the plasmalemma area). The mean proportion of the total volume occupied by the cytoplasm was calculated to be 9%. This value is consistent with the concept of a highly vacuolated cell. Other explanations for the biphasic relaxation curves are discussed. |
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Keywords: | Chara corallina turgor pressure relaxation tonoplast plasmalemma cytoplasmic volume hydraulic conductivity |
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