Separation of histones from contaminating ribosomal proteins by two-dimensional gel electrophoresis |
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| Authors: | A Savíc D Poccia |
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| Affiliation: | 1. Institute for Biological Research, Belgrade 11000, Yugoslavia;2. Department of Biology, State University of New York at Stony Brook, Stony Brook, New York 11794 USA |
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| Abstract: | ![]()
Sea urchin histones can be separated from ribosomal proteins by two-dimensional gel electrophoresis. Electrophoresis on Triton X-100/6 m urea gels in the first dimension results in preferential retardation of the histones, which then migrate more rapidly than ribosomal contaminants on SDS gel electrophoresis in the second dimension. The advantages and generality of the system are discussed. |
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| Keywords: | Address reprint requests to Dominic Poccia Department of Biology Amherst College Amherst Mass. 01002. |
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