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| 分枝杆菌中基因敲除操作工具研究进展 | |
| 引用本文： | Du QL,Fan XY,Mao JX,Xie JP. 分枝杆菌中基因敲除操作工具研究进展[J]. 遗传, 2012, 34(7): 857-862 |
| 作者姓名： | Du QL Fan XY Mao JX Xie JP |
| 作者单位： | 西南大学生命科学学院现代生物医药研究所,三峡库区生态环境与生物资源省部共建国家重点实验室培育基地 |
| 基金项目： | 国家重要传染病科技重大专项(编号:2008ZX10003-006,2012ZX10003003-004);国家自然科学基金项目(编号:81071316);教育部新世纪优秀人才支持计划(编号:NCET-2011);中央高校基本科研业务费专项资金(编号:XDJK2009A003,XDJK2011D006);西南大学研究生科技创新基金项目(编号:kb2010017)资助 |
| 摘 要： | 致病性分枝杆菌仍然是人类健康的重要威胁。相对于传统模式原核生物,在分枝杆菌中进行基因敲除一直是一个难题。文章介绍了分枝杆菌中用于基因敲除的转运载体和序列特异性重组系统的研究进展,并进一步介绍了新近发展的分枝杆菌重组工程在基因敲除中的应用。最后,对分枝杆菌基因敲除系统的发展历程进行了回顾,并对未来的发展方向进行了展望。 |
| 关 键 词： | 分枝杆菌 基因敲除 转运载体 序列特异重组 重组工程 |
Progression on genetic knockout tools in Mycobacterium |
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| Du Qin-Lin,Fan Xiang-Yu,Mao Jin-Xiao,Xie Jian-Ping. Progression on genetic knockout tools in Mycobacterium[J]. Hereditas, 2012, 34(7): 857-862 | |
| Authors: | Du Qin-Lin Fan Xiang-Yu Mao Jin-Xiao Xie Jian-Ping |
| Affiliation: | Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, School of Life Sciences, Southwest University, Chongqing 400715, China. duql@swu.edu.cn |
| Abstract: | Pathogenic mycobacteria were and remain a heavy burden to public health. Unfortunately, genetic manipulation including knockout technologies of Mycobacterium is difficult compared with other traditional model organisms. To overcome this obstacle, achievements in Mycobacterium knockout technologies were summarized, including delivery vector, sequence-specific recombination system, as well as the recently developed recombinogenic engineering and its application. The future for this tool innovation is also addressed. |
| Keywords: | Mycobacterium knockout delivery vector sequence-specific recombinase recombinogenic engineering |
| 本文献已被 CNKI PubMed 等数据库收录！ | |