Reconstituted human gingival epithelium: Nonsubmerged in vitro model |
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Authors: | Agnes Delcourt-Huard Anne Corlu Annick Joffre Henry Magloire Martine Bonnaure-Mallet |
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Institution: | 1. Equipe de Biologie Buccale, Université de Rennes, I, 2 place Pasteur, 35000, Rennes, France 2. Unité INSERM 49 H?pital Pontchaillou, France 3. Laboratoire d’Histophysiologie et Pathologie des tissus dentaires, Faculté d’Odontologie de Lyon, France
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Abstract: | Summary Many studies have shown that human gingival keratinocytes grown in submerged culture fail to attain optimal differentiation.
This study reports an in vitro culture system for oral gingival epithelial cells, in which they are grown at the air-liquid interface, on polycarbonate
inserts, in the presence of an NIH-3T3 feeder layer. This model was compared with two submerged culture methods for gingival
keratinocytes, on type I collagen gel and on an NIH-3T3 feeder layer. Transmission electron microscopy showed an advanced
level of stratification (over six layers of cells) for cultures grown at the air-liquid interface. Immunofluorescence and
electrophoretic patterns showed the presence of cytokeratins 10 and 11 in cytoskeletal protein extracts of these cultured
keratinocytes. In this air-liquid interface culture model, in the presence of NIH-3T3 feeder cells, keratinocytes can achieve
an advanced level of stratification and differentiation and a resemblance to in vivo gingiva. The obtention of a highly differentiated epithelium will permit in vitro pharmacological studies and studies on the biocompatability of certain alloys with the superficial periodontium; it will
also provide grafts for patients undergoing periodontal surgery. |
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Keywords: | gingiva human epithelial cells nonsubmerged culture |
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