Regulation of nitrile-hydratase synthesis in a Brevibacterium species |
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| Authors: | D Tourneix A Thiéry M Maestracci A Arnaud P Galzy |
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| Institution: | (1) Chaire de Génétique et Microbiologie, Ecole National Supérieure Agronomique de Montpellier, F-34060 Montpellier Cedex, France |
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| Abstract: | The regulation of nitrile-hydratase biosynthese was studied in Brevibacterium sp R 312. Enzyme biosynthesis was not influenced by any carbon and nitrogen source used in the growth medium. It was, however, repressed by amide and amide analogues. Acetamide repressed nitrile-hydratase biosynthesis and induced the wide-spectrum amidase. Therefore, it appeared reasonable to hypothesize a single repressor gene for the nitrile-hydratase/wide-spectrum amidase system. |
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