In vitro propagation of Clianthus formosus (Sturt's desert pea) an Australian native legume |
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Authors: | Acram M Taji Richard R Williams |
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Institution: | (1) Black Hill Native Flora Centre, 115 Maryvale Road, 5076 Athelstone, SA, Australia;(2) Department of Agronomy and Soil Science, University of New England, 2351 Armidale, N.S.W., Australia |
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Abstract: | Hypocotyl and cotyledon segments of Clianthus formosus were cultured on a modified deFossard medium M supplemented with cytokinins. 62% of cultures on medium with 20 μM BA produced
callus which subsequently gave rise to shoots. 40% of shoots excised from callus produced roots after transfer to auxin-rich
media (20 μM NAA or 10 μM IBA+10 μM NAA). Root production was enhanced following a 7-day dark treatment. 32% of nodes from
mature plants produced multiple shoots on 2 μM BA+2 μM KIN. 30% of these shoots rooted on medium without hormones. 70% of
rooted plantlets were successfully transferred to potting medium and glasshouse conditions. A period of cold treatment (10
days at 5°C) reduced vitrification from 68 to 22% of cultures. |
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Keywords: | Papilionaceae tissue culture vitrification root induction |
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