Liquid-based hybridization assay with real-time detection in miniaturized array platforms |
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Authors: | Guerasimova Anna Nyarsik Lajos Liu Jian-Ping Schwartz Regine Lange Matthias Lehrach Hans Janitz Michal |
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Institution: | Max Planck Institute for Molecular Genetics, Berlin, Germany. |
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Abstract: | An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption. |
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