Amino acid sequence and immunological properties of chachalaca egg white lysozyme |
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Authors: | Jacqueline Jollés FranÇOise Schoentgen Pierre Jollès Ellen M Prager Allan C Wilson |
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Institution: | (1) Laboratory of Proteins, University of Paris V, 45 rue des Saints-Pères, F-75270 Paris, Cedex 05, France;(2) Department of Biochemistry, University of California, 94720 Berkeley, Cal., USA |
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Abstract: | Summary The amino acid sequence of lysozyme c from chachalaca egg white was determined. Like other bird lysozymes c, that of the chachalaca has 129 amino acid residues. It differs from other avian lysozymes c by 27 to 31 amino acid substitutions as well as by being devoid of phenylalanine. It contains substitutions at 9 positions which are invariant in the other 7 bird lysozymes of known sequence. Although the chachalaca is classified zoologically in the order Galliformes, which includes chickens and other pheasant-like birds, its lysozyme differs more from those of pheasant-like birds than do the lysozymes c of ducks. Phylogenetic analysis of the sequence comparisons confirms that the lineage leading to chachalaca lysozyme c separated from that leading to other galliform lysozymes c before the duck lysozyme c lineage did. This indicates a contrast between protein evolution and evolution at the organismal level. Immunological comparison of chachalacalysozyme c with other lysozymes of known sequence provides further support for the proposal that immunological cross-reactivity is strongly dependent on degree of sequence resemblance among bird lysozymes.103rd communication on lysozymes from the Laboratory of P. Jollès. Supported in part by grants from C.N.R.S. (ER 102), I.N.S.E.R.M. (Groupe de recherche U-116), N.S.F. (GB-42028X), and N.I.H. (GM-21509). |
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Keywords: | Lysozyme Evolution Phylogenetic Analysis Birds Chachalaca Sequence-Immunology Correlation Micro-Complement Fixation |
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