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Potential antibiotic and anti-infective effects of rhodomyrtone from Rhodomyrtus tomentosa (Aiton) Hassk. on Streptococcus pyogenes as revealed by proteomics |
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| Authors: | Surasak LimsuwanAnne Hesseling-Meinders Supayang Piyawan VoravuthikunchaiJan Maarten van Dijl Oliver Kayser |
| Affiliation: | a Faculty of Traditional Thai Medicine and Natural Products Research Center, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand b Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborg 7, 9747 AG Groningen, The Netherlands c Department of Microbiology and Natural Products Research Center, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand d Department of Medical Microbiology, University Medical Center Groningen (UMCG) and University of Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands e Department of Bio- and Chemical Engineering, Technical University of Dortmund, Technical Biochemistry, Emil-Figge-Strasse 66-68, D-44227 Dortmund, Germany |
| Abstract: | Rhodomyrtone from Rhodomyrtus tomentosa (Aiton) Hassk. leaf extract has a strong antibacterial activity against the bacterial pathogen Streptococcus pyogenes. Our previous studies indicated that the bactericidal activity of rhodomyrtone might involve intracellular targets. In the present studies we followed a proteomics approach to investigate the mode of action of rhodomyrtone on S. pyogenes. For this purpose, S. pyogenes was cultivated in the presence of 0.39 μg/ml rhodomyrtone, which corresponds to 50% of the minimal inhibitory concentration. The results show that the amounts of various enzymes associated with important metabolic pathways were strongly affected, which is consistent with the growth-inhibiting effect of rhodomyrtone. Additionally, cells of S. pyogenes grown in the presence of rhodomyrtone produced reduced amounts of known virulence factors, such as the glyceraldehyde-3-phosphate dehydrogenase, the CAMP factor, and the streptococcal pyrogenic exotoxin C. Taken together, these findings indicate that rhodomyrtone has both antimicrobial and anti-infective activities, which make it an interesting candidate drug. |
| Keywords: | Rhodomyrtone Rhodomyrtus tomentosa Glycolysis Proteomics Streptococcus pyogenes Two-dimensional gel electrophoresis |
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