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The utilization of bromodeoxyuridine incorporation into DNA for the analysis of cellular kinetics |
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| Authors: | R Tice E L Schneider J M Rary |
| Institution: | 1. Department of Biology, School of Medicine, The Johns Hopkins University, Baltimore, MD 21205, USA;2. Department of Gynecology and Obstetrics, School of Medicine, The Johns Hopkins University, Baltimore, MD 21205, USA;3. Laboratory of Cellular and Comparative Physiology, Gerontology Research Centre, National Institute on Ageing, Baltimore City Hospital, Baltimore, MD 21224, USA |
| Abstract: | Recently developed differential staining techniques based on the incorporation of bromodeoxyuridine (BUdR) into DNA permits the unequivocal identification of metaphase cells which have replicated once, twice, and three or more times. This technique has the potential of being utilized in the examination of kinetics of dividing cell populations. This potential is examined in a phytohemagglutinin-stimulated lymphocyte system. Determinations of the effect of increasing concentrations of BUdR on the distribution of metaphase cells between different generation cycles reveals no inhibition of cellular kinetics below 35 μM. The ability to distinguish third generation metaphase cells from subsequent generations is examined through the determination of “labelled” centromeric regions. The applicability of this system to current cellular kinetics is discussed. |
| Keywords: | Address reprint request to: J M Rary Room 203 Biophysics Building The Johns Hopkins University School of Medicine Baltimore MD 21205 USA |
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