Additive and synergistic effects of Bacillus spp. isolates and essential oils on the control of phytopathogenic and saprophytic fungi from medicinal plants and marigold seeds |
| |
| Institution: | 1. Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia;2. Institute for Medicinal Plants Research “ Dr Josif Pan?i?”, Tadeu?a Ko??u?ka 1, 11000 Belgrade, Serbia;1. Université de Brest, EA 3882, Laboratoire Universitaire de Biodiversité et Écologie Microbienne, IBSAM, ESIAB, Technopôle Brest-Iroise, 29280 Plouzané, France;2. Lallemand SAS, 4 route de Beaupuy, 31180 Castelmaurou, France;3. Ottawa Research & Development Centre, Science & Technology Branch, Agriculture and Agri-Food Canada, 960 Carling Ave., Ottawa, ON K1A 0C6, Canada;1. Faculty of Agriculture, Pesticide Science Laboratory, Aristotle University of Thessaloniki, P.O.Box 1678, 54124 Thessaloniki, Greece;2. Faculty of Agricultural Development, Laboratory of Agricultural Pharmacology and Ecotoxicology, Democritus University of Thrace, 193 Pantazidou, 682 00 Orestias, Greece;1. University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Department for Algology, Mycology and Lichenology, Takovska 43, 11000 Belgrade, Serbia;2. University of Belgrade, Faculty of Mining and Geology, Department of Mineralogy, Crystallography, Petrology and Geochemistry, ?usina 7, 11000 Belgrade, Serbia;3. University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Department for Microbiology, Studentski trg 16, 11000 Belgrade, Serbia;1. Faculty of Agriculture, Forestry and Natural Environment, Laboratory of Plant Pathology, Aristotelian University of Thessaloniki, Thessaloniki, Greece;2. Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala Biocenter, Box 7026, SE-750 07, Uppsala, Sweden;1. Laboratory of Plant Improvement and Valorization of Agri-Resources, National Engineering School of Sfax, 3038, Sfax, Tunisia;2. Laboratory of Biotechnology and Plant Improvement, Center of Biotechnology of Sfax, 3018, Sfax, Tunisia |
| |
| Abstract: | Contamination of plants and seeds with microorganisms is one of the main problems in the production and distribution of various agricultural products, as well as raw herbal material for the preparation of herbal remedies. In targeting microbial contamination, among other bacteria, Bacillus species showed a significant capacity for biocontrol. The antifungal activity of 14 isolates of Bacillus spp. against 15 fungal isolates from medicinal plants was examined utilizing a dual plate assay. The strongest and broadest antagonistic activity against all fungi tested was exhibited by isolates SS-12.6 and SS-13.1 (from a 43% to 74% reduction in fungal growth), while isolates SS-39.1 and SS-39.3 were effective against the fewest fungus species and also had the weakest antifungal activity. The effect of a crude lipopeptide extract (CLE) of Bacillus sp. SS-12.6 was similar to that achieved by a dual culture with isolate SS-12.6, confirming that the antagonism was the result of the antifungal activities of lipopeptides. In addition, essential oils of thyme (0.55 mg/mL) and savory (0.32 mg/mL) in various combinations with the CLE of SS-12.6 were tested for antifungal activity, and additive and synergistic effects for some of the fungi were obtained. When testing the effect of CLE, oils (0.40 mg/mL for thyme oil and 0.21 mg/mL for savory oil) and combinations in situ on marigold seeds, a reduction of total fungal infection without an adverse effect on germination was accomplished by 6-h treatments with CLE of SS-12.6 (85% reduction of fungal infection and 63% germination), supernatant from liquid culture of SS-12.6 (more than 90% reduction of fungal infection with 69% seed germination) and combinations of CLE and savory oil (77% reduction of fungal infection and 62% seed germination) and CLE with thyme and savory oils (about 75% reduction of fungal infection with 69% seed germination). |
| |
| Keywords: | Antifungal activity Biocontrol Lipopeptides Thyme oil Savory oil Marigold seed Seed germination |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
