Cloning and Characterization of the Nucleoredoxin Gene That Encodes a Novel Nuclear Protein Related to Thioredoxin |
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| Authors: | Hisanori Kurooka Keizo Kato Shigeru Minoguchi Yoshinori Takahashi Joh-e Ikeda Sonoko Habu Nobutaka Osawa Arthur M. Buchberg Kazuo Moriwaki Hayase Shisa Tasuku Honjo |
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| Affiliation: | aDepartment of Medical Chemistry, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606, Japan;bDepartment of Molecular and Medical Genetics, Institute of Medical Science, School of Medicine, Tokai University, Isehara, Kanagawa, 259-11, Japan;cInstitute of Medical Science, Department of Immunology, School of Medicine, Tokai University, Isehara, Kanagawa, 259-11, Japan;dDepartment of Microbiology, School of Medicine, Kitasato University, Sagamihara, Kanagawa, 228, Japan;eDepartment of Microbiology and Immunology, Kimmel Cancer Site, Jefferson Medical College, Philadelphia, Pennsylvania, 19107;fGraduate University for Advanced Studies, Hayama, Kanagawa, 240-01, Japan;gDepartment of Pathology, Saitama Cancer Site Research Institute, Ina, Saitama, 362, Japan |
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| Abstract: | In a yeast artificial chromosome contig close to thenudelocus on mouse chromosome 11, we identified a novel gene, nucleoredoxin, that encodes a protein with similarity to the active site of thioredoxins. Nucleoredoxin is conserved between mammalian species, and two homologous genes were found inCaenorhabditis elegans.The nucleoredoxin transcripts are expressed in all adult tissues examined, but restricted to the nervous system and the limb buds in Day 10.5–11.5 embryos. The nucleoredoxin protein is predominantly localized in the nucleus of cells transfected with the nucleoredoxin expression construct. Since the bacterially expressed protein of nucleoredoxin showed oxidoreductase activity of the insulin disulfide bonds with kinetics similar to that of thioredoxin, it may be a redox regulator of the nuclear proteins, such as transcription factors. |
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