Genetic analysis of mannityl opine catabolism in octopine-type Agrobacterium tumefaciens strain 15955 |
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| Authors: | Yves Dessaux, Jacques Tempé Stephen K. Farrand |
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| Affiliation: | (1) Groupe de Recherche sur les Interactions entre Microorganismes et Plantes, Génétique et Amélioration des Plantes, INRA, F-91405 Orsay Cedex 05, France;(2) Institut de Microbiologie, Faculté des Sciences, CNRS, UA136, F-91405 Orsay Cedex 05, France;(3) Department of Microbiology, Loyola University Stritch School of Medicine, 2160 South First Avenue, 60153 Maywood, IL, USA;(4) Present address: Department of Plant Pathology, University of Illinois at Urbana/Champaign, 61801 Urbana, IL, USA |
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| Abstract: | Summary The genetic organization of functions responsible for mannityl opine catabolism of the Ti plasmid of Agrobacterium tumefaciens strain 15955 was investigated. A partial HindIII digest of pTi15955 was cloned into a broad host range cosmid and the clones obtained were tested for ability to confer mannityl opine degradation upon Agrobacterium. Inserts containing genes for catabolism of mannopinic acid, mannopine, agropine, and agropinic acid were obtained, spanning a segment of 43 kb on the Ti plasmid. Two clones conferring upon Agrobacterium the ability to catabolize the mannityl opines were mobilized to several Rhizobium sp., to Pseudomonas putida and P. fluorescens and to Escherichia coli. The catabolic functions were phenotypically expressed in all Rhizobium sp. tested, and in P. fluorescens, but not in P. putida or in E. coli. |
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| Keywords: | Agrobacterium tumefaciens Mannityl opine Ti plasmid Opine catabolism Catabolic plasmid |
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