Evolution of osmosensing signal transduction in Metazoa: stress-activated protein kinases p38 and JNK |
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Authors: | Böhm Markus Gamulin Vera Schröder Heinz C Müller Werner E G |
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Institution: | Institut für Physiologische Chemie, Abteilung für Angewandte Molekularbiologie, Universit?t, Duesbergweg 6, 55099 Mainz, Germany. |
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Abstract: | Sponges (Porifera) represent the most basal branch of the Metazoa alive today. We show that two central stress-activated protein kinases involved in the osmosensing pathway, p38 mitogen-activated protein kinase (MAPK) and JNK, can complement for the ancestral MAPK Hog1 in the yeast Saccharomyces cerevisiae. S. cerevisiae mutants lacking Hog1 (hog1-Delta 1) have been complemented with the sponge SDJNK and SDp38 genes. Western blotting has revealed that, after transformation, the hog1-Delta 1+ SDJNK(sense) and hog1-Delta 1+ SDp38(sense) clones express the sponge proteins. Functional studies have demonstrated that the complemented clones grow under hyperosmotic conditions (0.6 M NaCl). Furthermore, the expressed sponge kinases undergo phosphorylation in S. cerevisiae at 0.6 M NaCl. This report documents that the metazoan signal transduction kinases, p38 and JNK, which were originally derived from an common ancestor with yeast HOG1, have retained their function after their specification. |
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