Sucrose modifies ribosomal stability and conformation |
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Authors: | A M Reboud S Dubost J P Reboud |
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Institution: | Laboratoire de Biochimie Médicale, ERA CNRS No 399, U.E.R. Lyon Nord, Université Lyon I, 43 Bd du 11 novembre 1918, 69622 Villeurbanne, France |
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Abstract: | High concentrations of sucrose have a strong protective effect on heat-induced modifications of rat liver ribosomal subunits. They prevent to a large extent subunit inactivation, measured by poly (U)-dependent 14C] Phe tRNA binding (40S subunits) and puromycin reaction (60S subunits), subunit unfolding into light forms, and the release of both free and protein-complexed 5S RNA. They also increase the temperature at which subunits start to melt. Our data indicate that sucrose affects subunit conformation. |
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Keywords: | ribosome saccharose stabilité conformation ribosome sucrose stability conformation |
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