| 紫外-激光复合诱变选育天冬氨酸转氨酶高产菌株 |
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| 引用本文： | 薛正莲,江荣珍,杨超英,王洲,苏燕南.紫外-激光复合诱变选育天冬氨酸转氨酶高产菌株[J].氨基酸和生物资源,2010,32(4):46-49. |
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| 作者姓名： | 薛正莲 江荣珍 杨超英 王洲 苏燕南 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000;安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000;安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000;安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000;安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000 |
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| 摘 要： | 对天冬氨酸转氨酶产生菌大肠杆菌XJ-1原生质体进行紫外-激光复合诱变筛选,结果表明,复合诱变对该菌的原生质体有明显的致死作用。以致死率和正突变率为指标,确定了紫外和He-Ne激光照射的最佳时间分别为45 s和40min。在此条件下对大肠杆菌原生质体进行紫外-激光复合诱变,得到3株高产菌株,分别命名为XJ-1-45、XJ-1-86和XJ-1-99,酶活较出发菌株XJ-1分别提高了12.82%、17.37%和26.27%。传代培养表明突变株生产性能稳定。
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| 关 键 词： | 紫外线 He-Ne激光 天冬氨酸转氨酶 原生质体 复合诱变 |
Screening of High Aspartate Aminotransferase Producing Strains by UV and He - Ne Laser Irradiation |
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| XUE Zheng-lian,JIANG Rong-zhen,YANG Chao-ying,WANG Zhou,SU Yan-nan.Screening of High Aspartate Aminotransferase Producing Strains by UV and He - Ne Laser Irradiation[J].Amino Acids & Biotic Resources,2010,32(4):46-49. |
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| Authors: | XUE Zheng-lian JIANG Rong-zhen YANG Chao-ying WANG Zhou SU Yan-nan |
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| Institution: | XUE Zheng-lian,JIANG Rong-zhen,YANG Chao-ying,WANG Zhou,SU Yan-nan(Institute of Biologic & Chemical Engineering of Anhui Polytechnic University,Microbial Fermentation Engineering and Technology Research Center of Anhui Province,Wuhu 241000,China) |
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| Abstract: | Protoplast of E.coli XJ-1 producing aspartate aminotransferase was irradiated by UV and He-Ne laser compound mutagenesis.The result showed that compound mutagenesis had obvious lethal effect on E.coli XJ-1 protoplast.With the death rate and positive mutation as criteria,the optimum time for UV and He-Ne laser irradiation was 45s and 40 min respectively.Three high-yielding strains named XJ-1-45,XJ-1-86 and XJ-1-99 were obtained by 45s UV and 40min He-Ne laser compound mutagenesis,and activities of aspartate ... |
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| Keywords: | aspartate aminotransferase UV He-Ne laser protoplast compound mutagenesis |
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