白黎芦醇对胃癌SGC 一7 901 细胞V EGF 表达的影响

目的：探讨白藜芦醇（resveratrol，Res）在体外对胃癌SGC-7901细胞VEGF表达的影响。方法：体外培养胃癌SGC-7901细胞，MTT法检测白藜芦醇对SGC-7901细胞的增殖抑制作用，RT—PCR方法检测VEGFmRNA表达，免疫细胞化学检测VEGF蛋白的表达。结果：白藜芦醇呈时间剂量性抑制胃癌细胞SGC7901的增殖；胃癌SGC-7901细胞高水平表达VEGF，白藜芦醇能显著降低胃癌SGC-7901细胞VEGFmRNA和蛋白表达。结论：白藜芦醇可以下调胃癌SGC-7901细胞VEGF的表达，抑制胃癌细胞的增殖。

Effects of Resveratrol on the expression of VEGF in human gastric cancer SGC- 7901 cells

Objective:To investigate whether resveratrol could inhibit the vascular endothelial growth factor (VEGF) expression of human gastric cancer SGC-7901 cell in vitro.Methods:Human gastric cancer SGC-7901 cell line was cultured in vitro,proliferation inhihbitory effect was tested by MTT assay;VEGF mRNA was measured quantitatively by RT-PCR methold,Expression of VEGF protein in gastric cancer cells were measured by immunohistochemistry.Results:Resveratrol could inhibit the proliferation of SGC-7901 cells,with dose and time-related ef- fects;VEGF mRNA expressed in high level in SGC-7901 cells,in which,resveratrol significantly decreased the expression of VEGF mRNA and protein.Conclusion:Resveratrol could inhibit proliferation and lower the expression of VEGF in human gastric cancer SGC-7901 cell line in vitro.