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Medicago truncatula,a model plant for studying the molecular genetics of theRhizobium-legume symbiosis |
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| Authors: | David G Barker Sylvie Bianchi François Blondon Yvette Dattée Gérard Duc Sadi Essad Pascal Flament Philippe Gallusci Gérard Génier Pierre Guy Xavier Muel Jacques Tourneur Jean Dénarié Thierry Huguet |
| Institution: | 1. Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP 27, 31326, Castanet-Tolosan Cedex, France 2. Laboratoire d'Amélioration des Plantes, Université de Paris-Sud, Bat. 360, 91405, Orsay Cedex, France 4. Laboratoire du Phytotron, CNRS, BP 1, 91190, Gif sur Yvette, France 7. Station de Génétique et d'Amélioration des Plantes, INRA, BV 1540, 21034, Dijon Cedex, France 6. Station de Génétique et d'Amélioration des Plantes, INRA, Route de St. Cyr, 78026, Versailles Cedex, France 8. Station d'Amélioration des Plantes Fourragéres, INRA, 86600, Lusignan, France 9. Laboratoire de Biologie Cellulaire, INRA, Route de St. Cyr, 78026, Versailles Cedex, France |
| Abstract: | Medicago truncatula has all the characteristics required for a concerted analysis of nitrogen-fixing symbiosis withRhizobium using the tools of molecular biology, cellular biology and genetics.M. truncatula is a diploid and autogamous plant has a relatively small genome, and preliminary molecular analysis suggests that allelic heterozygosity is minimal compared with the cross-fertilising tetraploid alfalfa (Medicago sativa). TheM. truncatula cultivar Jemalong is nodulated by theRhizobium meliloti strain 2011, which has already served to define many of the bacterial genes involved in symbiosis with alfalfa. A genotype of Jemalong has been identified which can be regenerated after transformation byAgrobacterium, thus allowing the analysis ofin-vitro-modified genes in an homologous transgenic system. Finally, by virtue of the diploid, self-fertilising and genetically homogeneous character ofM. truncatula, it should be relatively straightforward to screen for recessive mutations in symbiotic genes, to carry out genetic analysis, and to construct an RFLP map for this plant. |
| Keywords: | symbiotic nitrogen fixation legume Medicago truncatula |
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