Lipid-bound sugars in malignant human breast tumors |
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Authors: | Ana Rosa Santa Cruza Luis A Quesada Allue Alberto Baldi |
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Institution: | 1. Instituto de Biologiá y Medicina Experimental, ‘Fundación Campomar’, Obligado 2490, 1428, Buenos Aires, Argentina 2. Instituto de Investigaciones Bioquímicas, ‘Fundación Campomar’, Obligado 2490, 1428, Buenos Aires, Argentina
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Abstract: | Microsomal preparations from malignant human breast tumors catalyzed the transfer of mannose and glucose from GDP-14C]-Man and UDP-14C]-Glc into lipid-linked sugars and glycoprotein-like substances. As judged by several criteria the obtained lipid-linked monosaccharides behaved as dolichyl phosphate mannose and dolichyl phosphate glucose whereas lipid-linked oligosaccharides behaved as polyprenyl diphosphate derivatives. The optimum conditions for mannosyl- and glucosyl-transfer reactions and the effect of dolichyl phosphate, detergent and EDTA on incubation mixture were described. |
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