Induction of chymoelastase (Pr1) of <Emphasis Type="Italic">Metarhizium anisopliae</Emphasis> and its role in causing mortality to mosquito larvae |
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Authors: | Suman Sundar Mohanty Kamaraju Raghavendra Aditya Prasad Dash |
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Institution: | (1) National Institute of Malaria Research (Indian Council of Medical Research), 22-Sham Nath Marg, Delhi, 110054, India;(2) National Institute of Malaria Research Field Station (Indian Council of Medical Research), Sector-5, Rourkela, Orissa, 769002, India |
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Abstract: | The efficacy of virulent strain Metarhizium anisopliae 892 obtained from Pyrausta nubilalis was evaluated against mosquito larvae. LC50 values of M. anisopliae 892 for Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti were compared. Metarhizium anisopliae 892 could cause approximately 50% mortality of C. quinquefasciatus 4 days post inoculation in the concentration of 3.48 × 103 conidia/ml. The production of cuticle degrading enzyme chymoelastase (Pr1) and trypsin like protease (Pr2) was compared in
the presence of inducers. There were significant differences in the production of Pr1 and Pr2 after addition of inducers i.e.
cuticles of the three mosquito. The cuticles of C. quinquefasciatus induced maximum Pr1 in the mycelia of M. anisopliae 892 than the rest of two mosquito cuticles during de-repression condition. The larvae of C. quinquefasciatus were more susceptible than the larvae of A. stephensi and A. aegypti against spores of M. anisopliae 892. The quantity of induction of Pr1 in the mycelia of M. anisopliae 892 was positively correlated with the mortality of mosquito larvae. Production of Pr1 and Pr2 was decreased when the inducers
were de-proteinated cuticles. The Pr2 induction of M. anisopliae 892 did not correlate with the mortality of mosquito larvae. From the observations of the present study it can concluded
that Pr1 is a responsible factor for the mortality of the mosquito larvae. This is the first report of Pr1 induction by mosquito
cuticle and its role in mosquito mortality. |
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Keywords: | Basal medium Cuticle Efficacy Entomopathogenic fungi De-repression |
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