PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli |
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| Authors: | Daniel K.Y. Solaiman |
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| Affiliation: | (1) Eastern Regional Research Center, US Department of Agriculture, ARS, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA |
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| Abstract: | A ca. 5.5-kb region of Pseudomonas resinovorans genome containing the polyhydroxyalkanoate (PHA) biosynthesis locus was sequenced. Three complete open-reading-frames (ORFs), i.e., phaC1Pr, phaZPr, and phaC2Pr, were identified. Using this sequence information, phaC1Pr was PCR-cloned from P. resinovorans genomic DNA and expressed in E. coli as shown by a Nile Red plate assay and gas chromatography/mass spectrometric analysis. |
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| Keywords: | gene expression PCR cloning pha genes polyhydroxyalkanoates Pseudomonas resinovorans |
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