Establishment and characterization of monoclonal antibodies against Chuzan virus K-47 |
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| Authors: | Nozomu Eto Koji Yamada Akiko Koga Sanetaka Shirahata Hiroki Murakami |
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| Institution: | (1) Department of Food Science and Technology, Faculty of Agriculture, Kyushu University 46-09, 6-10-1 Hakozaki, Higashi-ku, 812 Fukuoka, Japan;(2) Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University 46-09, 6-10-1 Hakozaki, Higashi-ku, 812 Fukuoka, Japan |
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| Abstract: | We established sixteen mouse monoclonal antibodies reactive to Chuzan virus K-47 strain using P3-X63-Ag8-U1 cells as fusion partner cells. Among them, CG53/2/4 recognized a 100K structural protein of the virus. The 100K antigen lost it's antigenicity for CG53/2/4 after mild periodate oxidation treatment, suggesting that the 100K viral antigen is a glycoprotein. In addition, CG53/2/4 neutralized the viral infectivity. This indicates that the 100K glycoprotein is essential for the infection of the virus. The other monoclonal antibodies reacted with a 41K antigen of the virus. Especially CG1/1 showed the highest reactivity to the virus. Forward step sandwich assay using CG1/1 and biotinylated CG53/2/4 could detect the virus at 10TCID50/ml. Therefore, these monoclonal antibodies can evantually predict the virus infection to the animals before their sideration. |
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| Keywords: | Chuzan virus monoclonal antibody neutralizing antibody forward sandwich assay |
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