On the phospholipid metabolism of glial cell primary cultures |
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Authors: | Heinrich Konrad Illig Brigitte Witter ProfDr Hildegard Debuch |
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Institution: | (1) Institut für Physiologische Chemie Lehrstuhl II, Universität Köln, Germany;(2) Instiut für Physiologische Chemie, Lehrstuhl II, Universität Köln, Joseph-Stelzmann-Str. 52, D-5000 Köln 41, Germany |
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Abstract: | Primary cultures prepared from newborn rat brain, consisted after 16 or 17 days mainly of astrocytes and of oligodendrocytes. 1-Alkenyl-sn-glycero-3-phosphoethanolamine (lysoplasmalogen) was used as substrate for studies on the metabolism of ethanolamine-glycerophospholipids. After 3 hr incubation two main products were observed: a) 1-alkenyl-2-acyl-sn-glycero-3-phosphoethanolamine (=ethanolamine plasmalogen) and b) 1-alkenyl-2-acyl-sn-glycero-3-phosphocholine (=choline plasmalogen). The acylation rate reached saturation at about 10 nmol substrate/mg cell protein with aV
max of 30 nmol×mg cell protein–1×3 hr–1. This acylated compound amounted to almost 60% of all radioactivity internalized, whereas the second product, choline plasmalogen, came to 20%. Unchanged substrate was found within the cells only in small amounts, even at maximum substrate internalization. These results were discussed in comparison with those obtained with 1-alkyl-sn-glycero-3-phosphoethanolamine under the same conditions (25). |
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