| 分离MEF和ES细胞的差速贴壁法研究(简报) |
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| 引用本文： | 徐超,黄华荣,俞宏,赵小立,陆永良,戴利成,姚行.分离MEF和ES细胞的差速贴壁法研究(简报)[J].分子细胞生物学报,2006,39(5):477-481. |
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| 作者姓名： | 徐超 黄华荣 俞宏 赵小立 陆永良 戴利成 姚行 |
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| 作者单位： | 浙江大学生命科学学院，浙江大学生命科学学院，浙江大学生命科学学院，浙江大学生命科学学院，浙江省湖州市中心医院，浙江省湖州市中心医院，浙江省湖州市中心医院 杭州 310012，杭州 310012，杭州 310012，杭州 310012，湖州 313000，湖州 313000，湖州 313000 |
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| 基金项目： | 浙江省重大项目(J20020579-30116)，湖州中心医院合作项目(H20010984-32536) |
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| 摘 要： | 胚胎干细胞的分化控制是胚胎干细胞研究的一个重要方面。由于常规的拟胚体诱导途径是在形成拟胚体后才开始进行诱导分化,受多种胚层的共同作用使得我们无法简便探索诱导分化的机制。而且用这种方法进行的诱导分化试验的结果检测比
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| 关 键 词： | 胚胎干细胞 饲养层细胞 差速贴壁 拟胚体 |
| 收稿时间： | 2006-02-05 |
| 修稿时间： | 2006-07-05 |
THE STUDY OF SEPARATING MURINE EMBRYONIC FIBROBLAST FROM EMBRYONIC STEM CELLS BY THE DIFFERENTIAL ADHESION METHOD |
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| XU Chao,HUANG Hua Rong,YU Hong,ZHAO Xiao Li,LU Yong Liang,DAI Li Cheng,YAO Xing.THE STUDY OF SEPARATING MURINE EMBRYONIC FIBROBLAST FROM EMBRYONIC STEM CELLS BY THE DIFFERENTIAL ADHESION METHOD[J].Journal of Molecular Cell Biology,2006,39(5):477-481. |
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| Authors: | XU Chao HUANG Hua Rong YU Hong ZHAO Xiao Li LU Yong Liang DAI Li Cheng YAO Xing |
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| Institution: | College Of Life Sciences,Zhenjiang University, Hangzhou 310012 |
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| Abstract: | This study has established a simple method of separating murine embryonic fibroblasts (MEF) from embryonic stem cells (ES cells). The adhesion rates of MEF and ES cells were compared to find out the opitimum time of removing MEF . The result shows that 1.5h and 0.5% gelatin concentration is the opitimum condition of removing MEF. Furthermore,the ES cells which have been purified have the same plating efficiency and the ability of three germ layers differentiation as the unpurified ES cells. After differential adhension,there is strong ALP activity in ES cells. All the results show that the purified ES cells are still in the state of undifferentiation and maintain the pluripotent. |
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| Keywords: | Embryonic stem cells Murine embryonic fibroblasts Differential adhesion Embryonic body |
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