Arabidopsis cyclin D2 expressed in rice forms a functional cyclin-dependent kinase complex that enhances seedling growth |
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Authors: | Se-Jun Oh Su-Jung Kim Youn Shic Kim Su-Hyun Park Sun-Hwa Ha Ju-Kon Kim |
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Institution: | (1) School of Biotechnology and Environmental Engineering, Myongji University, Yongin, 449-728, South Korea;(2) National Institute of Agricultural Biotechnology, RDA, Suwon, 441-770, South Korea |
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Abstract: | D-class cyclins play important roles in controlling the cell cycle in development and in response to external signals by forming
the regulatory subunit of cyclin-dependent kinase (CDK) complexes. To evaluate the effects of D-class cyclins in transgenic
rice plants, Arabidopsis cyclin D2 gene (CycD2) was linked to the maize ubiquitin1 promoter (Ubi1) and introduced into rice by the Agrobacterium-mediated transformation method. Genomic deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and Western blot hybridizations
of the Ubi1:CycD2 plants revealed copy number of transgene and its increased expression in leaf and callus cells at messenger RNA (mRNA) and/or
protein levels. The H1 kinase assay using the immunoprecipitates of protein extracts from the Ubi1:CycD2 plants and nontransgenic controls demonstrated that the introduced Arabidopsis CycD2 forms a functional CycD2/CDK complex
with an unidentified CDK of rice. Shoot and root growth was enhanced in the Ubi1:CycD2 seedlings compared with nontransgenic controls, together, suggesting that Arabidopsis cyclin D2 interacts with a rice cyclin-dependent
kinase, consequently enhancing seedling growth. |
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Keywords: | Cyclins Arabidopsis CycD2 Cyclin-dependent kinase Transgenic rice |
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