A mutant of Halobacterium halobium with constitutive production of bacteriorhodopsin |
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Authors: | Guadalupe Juez F Rodriguez-Valera |
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Institution: | Departamento de Microbiologia, Facultad de Medicina, Universidad de Alicante, Alicante, Spain |
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Abstract: | Abstract A purple mutant of Halobacterium halobium was isolated in a previous study. The 'in vitro' absorption spectra of the cells gave a broad shoulder around 570 nm. The amounts of bacteriorhodopsin were high under any growth condition (including aerobic) inhibitory for the wild-type strain. The mutant grew faster under illuminated microaerophilic conditions and showed faster proton extrusion than the wild-type strain. This evidence shows that the mutant has a constitutive bacteriorhodopsin production not influenced by the oxygen concentration in the medium. However, some stimulation by light was found. |
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Keywords: | Archaebacteria genetics regulation of bacteriorhodopsin production purple membrane |
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