马肝醇脱氢酶催化有机硅酮不对称还原反应动力学

探讨了马肝醇脱氢酶(HLADH)催化三甲基硅乙酮及其碳结构类似物不对称还原反应动力学.结果表明，在酶浓度低于150 mg/L时，底物浓度与反应初速度的关系符合米氏动力学方程；HLADH催化三甲基硅乙酮不对称还原反应的K_m、v_max和E_a分别为2.67 mmol/L、0.118 mmol/(L·min·mg)和37 kJ/mol， 其碳结构类似物的相应值分别为3.56 mmol/L、0.084 mmol/(L·min·mg)和61 kJ/mol.

Study on Kinetics of Asymmetric Reduction of Organosilyl Ketone Catalyzed by Horse Liver Alcohol Dehydrogenase

The kinetics of asymmetric reduction of acetyltrimethylsilane and its carbon analogue catatlyzed by horse liver alcohol dehydrogenase were explored. It has been found that the relation between substrate concentration and initial reaction rate was in accordance with the Michaelis-Menten equation when enzyme concentration was below 150 mg/L and the K_m, v_max and E_a of HLADH-catalyzed asymmetric reduction of acetyltrimethylsilane were 2.67 mmol/L, 0.118 mmol/(L·min·mg) and 37 kJ/mol respectively, while the corresponding parameters for its carbon analogue were 3.56 mmol/L, 0.084 mmol/(L·min·mg) and 61 kJ/mol.