| 外源性VEGF对结肠癌细胞SW480蛋白质变化的影响 |
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| 引用本文： | 王学虎,傅仲学,赵渝,谌伟,吴星烨.外源性VEGF对结肠癌细胞SW480蛋白质变化的影响[J].生命科学研究,2010,14(1):62-66. |
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| 作者姓名： | 王学虎 傅仲学 赵渝 谌伟 吴星烨 |
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| 作者单位： | 重庆医科大学,附属第一医院普通外科,中国重庆,400016 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 利用RT-PCR发现结肠癌细胞存在血管内皮生长因子(VEGF)及其受体(VEGFR)的表达,随后采用外源性VEGF对结肠癌细胞SW480进行体外刺激,流式细胞仪检测到结肠癌细胞刺激后快速进入分裂期,同时提取VEGF作用前后的结肠癌细胞总蛋白,蛋白质组学研究,发现存在明显的蛋白质变化,其中鉴定出10个差异蛋白质.提示外源性VEGF可以促进结肠癌细胞增殖并对肿瘤细胞蛋白质组产生明显的影响.
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| 关 键 词： | 血管内皮生长因子 蛋白质组 双向凝胶电泳 结肠癌 |
Protein Changes in Colon Cancer Cells After VEGF Stimulation Using Proteomic Technique |
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| WANG Xue-hu,FU Zhong-xue,ZHAO Yu,SHEN Wei,WU Xing-ye.Protein Changes in Colon Cancer Cells After VEGF Stimulation Using Proteomic Technique[J].Life Science Research,2010,14(1):62-66. |
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| Authors: | WANG Xue-hu FU Zhong-xue ZHAO Yu SHEN Wei WU Xing-ye |
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| Institution: | WANG Xue-hu,FU Zhong-xue,ZHAO Yu,SHEN Wei,WU Xing-ye (General Surgery,the First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | VEGF and VEGFR expressed in colon cancer cells detected by RT-PCR.Then,the colon cancer cells were treated with exogenous VEGF and we found that VEGF could promote cancer cells disintegrate detected by flow cytometry.Many protein spots separated by two-dimensional gel electrophoresis changed after VEGF stimulated and 10 proteins were identified by peptide mass fingerprinting.It indicated that colon cancer protein changes obviously after VEGF stimulated. |
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| Keywords: | vascular endothelial growth factor proteome two-dimensional gel electrophoresis colon cancer |
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