Olive oil addition in insect cell cultivation |
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| Authors: | Hwai-Shen Liu Yu-Chi Wang Tzyy-Wen Chiou |
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| Institution: | (1) Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.;(2) Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan, R.O.C. |
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| Abstract: | Adding olive oil to an insect cell (Spodoptera frugiperda) cultivation with a TNM-FH medium enhanced cell growth. In the static cultivation, growth with 0.5% oil increased viable cell density by 32%, while cultivation in spinner flasks agitated at 260 rpm increased by 64%. With a gradual increase of agitation from 60 rpm to 500 rpm, the viable cell density was 81% higher than that without the olive oil supplement. |
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