Elevated levels of oxidative DNA damage activate p53 and caspases in brain of ayu with aging |
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| Authors: | R. Nagasaka N. Okamoto H. Ushio |
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| Affiliation: | Laboratory of Fish Physiology, Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato, Tokyo;;Laboratory of Marine Biochemistry, Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan |
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| Abstract: | ![]()
It is well known that ayu (Plecoglossus altivelis) die after spawning. Their lifespan is known to be only 1 year; possibly one contributing factor to post‐spawning mortality in ayu is the enhanced oxidative stress, probably inducing DNA damage and subsequent DNA repair systems (i.e. phosphorylated p53), which in turn may cause apoptosis and a shortened lifespan. To examine this possibility, we surveyed p53 and its phosphorylation state, oxidative DNA damage by measuring the levels of 8‐hydroxy‐2′‐deoxyguanosine, and the induction of apoptosis by measuring levels of caspase‐3, ‐9/6 in the brain at different stages. Accumulation of oxidative stress in brain DNA was accompanied by caspase‐3, ‐9/6, and stimulates p53 through the phosphorylation of this p53 (specifically residue Ser 15) in ayu brain with aging. |
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