MiR-302/367在体细胞向多能性干细胞重编程中的研究

MicroRNA-302/367(miR-302/367)发现于2003年,是一类长度在21～22 nt的miRNA簇,与多能性干细胞自我更新及多向分化有重要关系.在体细胞向多能性干细胞重编程中具有重要作用. miR-302/367簇中各miRNA具有相对保守的种子区及靶基因,主要通过抑制靶基因蛋白质的翻译，从而促进间质-上皮转化（mesenchymal epithelial transition, MET）、抑制细胞周期、调控细胞分化相关基因及表观遗传水平等方式促进体细胞向多能性细胞重编程.本文对miR 302/367的发现、结构、miR 302/367在多能性细胞中的作用及在体细胞向多能性干细胞重编程中的作用及其机理等做一综述.

Roles of MiR-302/367 in the Reprogramming Somatic Cells into nduced Pluripotent Stem Cells

MiR-302/367， found in 2003, were a class of miRNAs with a length of 21～22 nt, act importantly in self renewal and multiple differentiation potential maintaine in pluripotent stem cells, also play an important role in somatic cell reprogramming. Each miRNA of miR 302/367 owns a relative conserve seed region, mainly through inhibiting target gene translation to promote mesenchymal epithelial transition (MET), inhibiting cell cycle, regulating differentiation associated genes- expression and epigenetic modification, finally acts well in somatic cell reprogramming. This paper reviews the discovery, structure of miR 302/367, their association with pluripotency and roles in somatic cell reprogramming and uncovers the molecular mechanism during the process.