Overproduction of Serratia marcescens metalloprotease (SMP) from the recombinant Serratia marcescens strains |
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| Authors: | Ki Seok Kim Koon Sig Park Si Myung Byun Jae Gu Pan Yong Chul Shin |
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| Affiliation: | (1) Department of Microbiology, College of Natural Sciences, Gyeongsang National University, 660-701 Chinju, Republic of Korea;(2) Department of Life Science, Korea Advanced Institute of Science and Technology(KAIST), 305-701 Taejeon, Republic of Korea;(3) Biochemical Process Laboratory, Genetic Engineering Research Institute, KIST, 305-606 Taejon, Republic of Korea |
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| Abstract: | Summary To overproduce Serratia marcescens metalloprotease(SMP), various recombinant plasmids encoding SMP gene were constructed and the SMP productivities from the recombinant S. marcescens strains were examined. The recombinant S. marcescens strains indispensably required proteinaceous substrates such as casein for the extracellular production of SMP. We obtained maximum 9,100U/ml of SMP from the culture supernatant of S. marcescens ATCC27117 containing a regulatory plasmid pTSP2 encoding SMP gene fused with a strong trc99a promoter and its repressor gene lacIq, which is about 23 times higher than that of wild type strain. SMP produced from the recombinant S. marcescens(pTSP2) was 88.3% of total extracellular proteins. |
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