Epigenetic Control of Salmonella enterica O-Antigen Chain Length: A Tradeoff between Virulence and Bacteriophage Resistance |
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| Authors: | Ignacio Cota María Antonia Sánchez-Romero Sara B Hernández M Graciela Pucciarelli Francisco García-del Portillo Josep Casadesús |
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| Institution: | 1. Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.; 2. Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.; 3. Departamento de Biología Molecular, Universidad Autónoma de Madrid, Centro de Biología Molecular Severo Ochoa (CBMSO-CSIC), Madrid, Spain.; Seoul National University, REPUBLIC OF KOREA, |
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| Abstract: | The Salmonella enterica opvAB operon is a horizontally-acquired locus that undergoes phase variation under Dam methylation control. The OpvA and OpvB proteins form intertwining ribbons in the inner membrane. Synthesis of OpvA and OpvB alters lipopolysaccharide O-antigen chain length and confers resistance to bacteriophages 9NA (Siphoviridae), Det7 (Myoviridae), and P22 (Podoviridae). These phages use the O-antigen as receptor. Because opvAB undergoes phase variation, S. enterica cultures contain subpopulations of opvAB
OFF and opvAB
ON cells. In the presence of a bacteriophage that uses the O-antigen as receptor, the opvAB
OFF subpopulation is killed and the opvAB
ON subpopulation is selected. Acquisition of phage resistance by phase variation of O-antigen chain length requires a payoff: opvAB expression reduces Salmonella virulence. However, phase variation permits resuscitation of the opvAB
OFF subpopulation as soon as phage challenge ceases. Phenotypic heterogeneity generated by opvAB phase variation thus preadapts Salmonella to survive phage challenge with a fitness cost that is transient only. |
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