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Quaternary Structure of Nucleoside Diphosphate Kinases |
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| Authors: | Ioan Lascu Anna Giartosio Stéphane Ransac Muriel Erent |
| Institution: | (1) Institut de Biochimie et Génétique Cellulaires, UMR 5095, University of Bordeaux-2 and CNRS, 33077 Bordeaux, France;(2) Istituto Pasteur-Fondazione Cenci-Bolognetti and Dipartimento di Scienze Biochimiche, Università La Sapienza, 00185 Roma, Italy;(3) Institut de Biochimie et Génétique Cellulaires, UMR 5095, University of Bordeaux-2 and CNRS, 33077 Bordeaux, France;(4) National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK |
| Abstract: | Nucleoside (NDP) diphosphate kinases are oligomeric enzymes. Most are hexameric, but somebacterial enzymes are tetrameric. Hexamers and tetramers are constructed by assemblingidentical dimers. The hexameric structure is important for protein stability, as demonstratedby studies with natural mutants (the Killer-of-prune mutant ofDrosophila NDP kinase andthe S120G mutant of the human NDP kinase A in neuroblastomas) and with mutants obtainedby site-directed mutagenesis. It is also essential for enzymic activity. The function of the tetrameric structure is unclear. |
| Keywords: | NDP kinase subunit interaction quaternary structure evolution mixed oligomers Dictyostelium |
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